Lime peel extract (Citrus Aurantifolia) inhibit the growth of bacteria Lactobacillus Acidophilus in childhood dental caries

Abstract

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Caries occur due to the presence of pathogenic microorganisms such as Lactobacillus Acidophilus bacteria. One way to prevent caries is to inhibit and kill Lactobacillus Acidophilus bacteria. This study used the dilution method to determine the inhibition and killing power of lime peel extract (Citrus Aurantifolia) against Lactobacillus Acidophilus bacteria, lime peel extract obtained by maceration method and made with concentrations of 100%, 50%, 25%, 12.5%, 6.25%, 3.125%, 1.56% and 0.78%. Based on the results of the study, it was found that lime peel extract with a concentration of 1.56% proved to be able to inhibit bacterial growth, at concentrations of 100%, 50%, 25%, 12.5%, 6.25% and 3.125% showed no growth of bacteria on the media. In the normality test used the Shapiro-Wilk method, the results obtained by the value of ρ (Sig.) > 0.05 so that the data is said to be normally distributed. Followed by the homogeneity test using the Levene Test and the value of ρ (Sig.) = 0.001 <0.05 so that the data is declared to be inhomogeneous. The Kruskall-Wallis test to determine the differences between the treatment groups and the value of ρ (Sig.) < 0.05, which means there was a significant difference and continued with the Mann-Whitney test to determine which groups were significantly different. The content of lime peel extract that affects the inhibition and killing power of bacteria are flavonoids, tannins, essential oils, saponins and triterpenoids which have antibacterial properties and are able to inhibit and kill Lactobacillus Acidophilus bacteria.