Inhibitory power test of moringa oleifera lamk against Actinobacillus Actinomycetemcomitans and its toxicity test to SGOT SGPT in rat

Abstract

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This study was to test the effectiveness of Moringa seed oil extract in inhibiting the growth of Actinobacillus Actinomycetemcomitans bacteria and to test the toxicity of Moringa seed oil extract. This type of research is experimental laboratory with post test only control group design. Pure Moringa leaf seed oil concentrations of 25%, 50% and 75% were used as experimental materials and the bacteria Actinobacillus Actinomycetemcomitans as test samples. The inhibition test used the Kirby-Bauer method while the SGOPT and SPGT examinations were used to measure the level of toxicity. The largest inhibition zone was found at a concentration of 75% at 4.73 and the smallest at a concentration of 25% at 2.23, while for positive control the inhibition zone was 16.44 and negative control was 0.10. Toxicity test shows that the safe dose that can be used is no more than 1250mg/kg, while toxicity occurs at a dose of 2500-5000 mg/kg. The results of statistical tests also showed a significant difference between the control and test groups of 2500 and 5000mg/kg. Conclusion : Moringa fruit seed oil (Moringa Olefiera L) has the greatest inhibitory power at a concentration of 75% with a dose of not more than 1250 mg/kg.